Training Example: JPK Instruments (Bruker BioAFM) – Review the Data, Give Your Score & Compare to the Real AI Evaluation

Microscopes
[H1] Life Sciences Research AFMs

Providing unique insights into life sciences research with previously inaccessible data, including physical structure, biochemical interactions, and mechanical properties

[H2] Advanced Imaging

Bruker has been helping biologists discover, understand, publish, and advance their knowledge of biological systems ever since our introduction of TappingMode™ expanded AFM use into life sciences research. In addition to general-purpose BioAFM systems specifically designed for life sciences research and suitable for a broad range of applications, Bruker offers a variety of specialized systems utilizing proprietary AFM features. These instruments include BioAFMs for high-speed imaging to support the investigation of dynamic processes; BioAFMs optimized for the investigation of cells and tissues, even uneven or highly corrugated samples; and BioAFMs for single-molecule applications ranging from single-molecule property characterization to the investigation of conformational changes at the single-molecule level, intra-molecular dynamics, and single-molecule spectroscopy (SMFS).
These instruments deliver the highest quality results for quantitative live-cell mechanical property mapping, highest resolution molecular and cellular imaging, and fast scanning of dynamic biological processes, as well as seamless integration of AFM with advanced optical microscopy techniques.

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter.

Learn More About BioAFM Technology and Capabilities

[H4] AFM for Life Science Research E-Book
Learn more about BioAFM technology and get practical tips and other information valuable to both AFM users and those not familiar with the technique.

Download the E-Book

[H4] Browse All BioAFM Resources
Explore our extensive offering of resources, including a brand new poster featuring the NanoWizard AFM platform, application and technical notes, research highlights, and more.

Learn More

Products
[H2] Find the Best BioAFM for Your Application

[H3] General-Purpose BioAFMs

[H4] NEW NanoWizard PURE
Streamlined design with best-in-class performance and flexibility.

Read More

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[H3] BioAFMs for High-Speed Imaging

[H4] NEW NanoWizard ULTRA Speed 3
Fast automated imaging at 1,400 lines per second and seamless integration into advanced optical microscopy and super-resolution techniques.

Read More

[IMG: JPK NanoRacer]
[H4] NanoRacer High-Speed AFM
True high-speed imaging at 50 frames and 5000 lines per second and exceptional usability for following molecular dynamics in real-time.

Mehr

[IMG: Dimension FastScan Bio]
[H4] Dimension FastScan Bio
FastScan speeds and superior AFM ease of use for life science

Mehr

[H3] BioAFMs for Cells and Tissues

[H4] NEW CellWizard Stage
Automated, AI-guided, multi-chamber in vitro imaging and nanomechanical analysis.

READ MORE

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[IMG: CellHesion 300 - automated, quantitative single-cell and tissue mechanics]
[H4] CellHesion 300
Automated platform for measuring cell-cell, cell-tissue, and cell-substrate interactions with single-molecule sensitivity.

Mehr

[H3] BioAFMs for Single-Molecule Applications

[IMG: ForceRobot® 400 Automated Force Spectroscope]
[H4] ForceRobot 400
For single molecule applications. Automated force spectroscope with optional temperature control and fluidics system.

Mehr

[H3] Related Technologies

[IMG: Optical Tweezers]
[H4] Optical Tweezers
Force-sensing optical tweezers and optical trapping

Mehr

Accessories and System Options
[H2] Customize Your BioAFM System

[H3]
Accessories to extend your BioAFM capabilities
Bruker offers an extensive range of BioAFM system add-ons, accessories, and modes to deliver maximum experimental and sample control, superior versatility, and enhanced useability. These options extend the range of applications and experiments supported by Bruker BioAFMs far beyond what is possible with any other BioAFM system on the market today.
Available options include optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions, and more.

Browse our online accessories database or download our accessories handbook to learn more.

[IMG: Options and add-ons for Bruker]
[H4] BioAFM Accessories Handbook [PDF]
Learn more about options and add-ons for Bruker BioAFMs, including 100+ accessories and 30 modes.

Download Now

[H2] Explore Our Virtual Showroom

BioAFM FAQs
[H2] Frequently Asked Questions

BioAFM is an increasingly important tool in biological and biomedical studies due to its very high resolution and the possibility of conducting experiments with live cells under physiologically relevant and ambient conditions, even in liquid. BioAFM also provides nanometer-resolution surface mapping for many mechanical and electrical properties, such as elasticity, stiffness, conductivity and surface potential. Bruker BioAFM technology is enabling life science researchers to investigate how these properties impact on cellular functions, including communication, signaling, cell division and differentiation, and even tumor metastasis and infection.

What is a BioAFM?

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter. BioAFM instruments can measure soft, fragile, and challenging samples — including single molecules, nucleic acids, bacteria, living cells, and tissues — under near-physiological conditions without damaging their structures. These instruments are uniquely capable of performing non-invasive measurements on biological samples and label-free measurements in liquid.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

How are BioAFMs specifically adapted for biological studies?

[H4] How are BioAFMs specifically adapted for biological studies?
BioAFMs have specialized sample and cantilever holders, stages, and measurement modes specifically designed for life science samples and experiments. Bruker BioAFM instruments also can be uniquely configured with a large selection of optional accessories and advanced modes, extending sample compatibility and measurement capabilities to include even the most challenging soft matter and biological samples.
Unique features and capabilities of Bruker BioAFM instruments include:
High-speed AFM with up to 50 frames per second enables the visualization of molecular dynamics.Precise force control prevents damage of delicate biological samples.Fully integrated correlated analysis with a wide range of advanced optical techniques.Data analysis methods for soft samples (e.g., contact point imaging).Large z range (>100µm) for measuring challenging biological samples with steep, highly corrugated surfaces, and cell adhesion experiments.Advanced automation features (e.g., detector alignment, creation of experiment workflows, and adjustment of scanning parameters) enable self-regulating measurement routines that maximize throughput and improve accuracy.Optical Tiling software feature for navigating around large samples.Cantilever holders that can be cleaned and autoclaved, providing direct access to biohazards in biosafety laboratories and allowing sample preparation, loading, experiments, and disposal to be performed in the BSL facility.Accessories for investigating a diverse range of biological and soft matter samples on substrates of various sizes, shapes, and materials (e.g., Petri dishes, coverslips, biochips, etc.) and in different environmental conditions (e.g., atmosphere, temperature, gas, pH-value, etc.).Automated, large area, multiparametric characterization of densely packed cell layers and highly corrugated tissue samples without microtome cutting.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

Browse All BioAFM Accessories

What are the advantages of using atomic force microscopy for biological research?

[H4] What are the advantages of using a BioAFM?
Atomic force microscopy provides 3D images of surface features and topography. BioAFMs extend and enhance the technique, optimizing it to address the unique needs and challenges of biological research and providing distinct advantages over other methods. Key advantages include:
High spatiotemporal resolution: Investigate morphology/surface structure at sub-molecular resolution and interactions in the piconewton range.AFM in liquids and environmental control: Study samples in liquid and under near-physiological conditions — ideal for the investigation of living cells in medium — capabilities that are unique to atomic force microscopy.Label-free and non-invasive investigation of living cells and delicate biological samples.Easy combination with other advanced optical microscopy techniques for correlated measurements and complementary datasets that provide a more comprehensive insight into biological processes.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

What are the advantages of automated BioAFM measurements in biological experiments?

[H4] What are the advantages of automated BioAFM measurements?
Bruker BioAFMs feature several specialized software functions and capabilities designed to support high-performance AFM-based investigation of biological samples and soft matter. Automated instrument procedures (e.g., alignment, operation, calibration), measurement routines, and data analysis capabilities make it easier to set up and run experiments while also improving the accuracy and reproducibility of results.
Advanced automation features include:
ExperimentPlanner: Predefinition of settings and parameters allows complex experiments to run automatically.ExperimentControl: Remote monitoring of long-term lab experiments via a browser on any device. Together with ExperimentPlanner, these features open a host of new possibilities for long-term, self-regulating experiment series that reflect “real-life” conditions. This alleviates long and repetitive experiments and gives researchers the freedom to oversee and control their experiments remotely.SmartMapping: The novel SmartMapping feature allows the flexible selection of multiple, user-defined 2D force maps. Numerous regions of interest (ROI) can be selected in advance and examined automatically, enabling the systematic study of large sample areas. Automated large-area, multi-region imaging (by combining DirectTiling, DirectOverlay 2, and MultiScan software features) extends the optical viewing field. Perfect optical integration enables direct correlation of AFM and optical data.
These features extend and enhance the capabilities of Bruker BioAFM systems in order to:
Increase throughput and productivity.Produce statistically relevant datasets.Reveal parameter correlation and cross-correlation via automated cycling through of parameters.Allow unattended, long term, self-regulating experiment series.Enable remote monitoring of long-term lab experiments.
The improved throughput, standardized batch analysis routines, and statistically relevant datasets generated by these types of automated features are essential in biological research, particularly in nanomedical and clinical fields.

RELATED LINKS:

[WATCH] Automated AFM and Applications in Mechanobiology and Life Science Research

Browse our Automated BioAFM Accessories

How and why do you integrate an AFM with an optical/fluorescence microscope?

[H4] How and why do you integrate a BioAFM with an optical/fluorescence microscope?
A major advantage of Bruker’s BioAFM instrumentation is that they can be easily combined with advanced optical microscopy techniques, e.g., fluorescence or STED microscopy, for correlated measurements and complementary datasets.
Our BioAFMs can be easily combined with advanced optical imaging techniques by using a specific AFM stage that is compatible with most commercially available inverse and confocal optical microscopes. The AFM head is placed on the stage and software, such as Bruker’s DirectOverlay feature, enables colocalization of the optical and AFM images and direct correlation of the AFM and optical data. Transferring the sample between setups is not necessary and a wide range of camera and detector types are supported. Easy optical image import, advanced calibration algorithms, and visualization routines facilitate accurate navigation on and around the sample, providing multidimensional sample characterization in a single experiment. Compatible techniques include epifluorescence, confocal, phase contrast, and super-resolution microscopy techniques (STED, TIRF, STORM).
The ability to obtain real-time, correlative data sets is particularly relevant in life science research because it:
Allows researchers to study the topography of biological samples using AFM while simultaneously observing fluorescently labeled cellular components; andEnables the multiparametric observation of in-situ dynamics, such as single-molecule protein dynamics, protein folding, receptor-ligand interactions, and mechanosensitive signaling pathways.

RELATED LINKS:

[WATC

Microscopes
[H1] Life Sciences Research AFMs

Providing unique insights into life sciences research with previously inaccessible data, including physical structure, biochemical interactions, and mechanical properties

[H2] Advanced Imaging

Bruker has been helping biologists discover, understand, publish, and advance their knowledge of biological systems ever since our introduction of TappingMode™ expanded AFM use into life sciences research. In addition to general-purpose BioAFM systems specifically designed for life sciences research and suitable for a broad range of applications, Bruker offers a variety of specialized systems utilizing proprietary AFM features. These instruments include BioAFMs for high-speed imaging to support the investigation of dynamic processes; BioAFMs optimized for the investigation of cells and tissues, even uneven or highly corrugated samples; and BioAFMs for single-molecule applications ranging from single-molecule property characterization to the investigation of conformational changes at the single-molecule level, intra-molecular dynamics, and single-molecule spectroscopy (SMFS).
These instruments deliver the highest quality results for quantitative live-cell mechanical property mapping, highest resolution molecular and cellular imaging, and fast scanning of dynamic biological processes, as well as seamless integration of AFM with advanced optical microscopy techniques.

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter.

Learn More About BioAFM Technology and Capabilities

[H4] AFM for Life Science Research E-Book
Learn more about BioAFM technology and get practical tips and other information valuable to both AFM users and those not familiar with the technique.

Download the E-Book

[H4] Browse All BioAFM Resources
Explore our extensive offering of resources, including a brand new poster featuring the NanoWizard AFM platform, application and technical notes, research highlights, and more.

Learn More

Products
[H2] Find the Best BioAFM for Your Application

[H3] General-Purpose BioAFMs

[H4] NEW NanoWizard PURE
Streamlined design with best-in-class performance and flexibility.

Read More

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[H3] BioAFMs for High-Speed Imaging

[H4] NEW NanoWizard ULTRA Speed 3
Fast automated imaging at 1,400 lines per second and seamless integration into advanced optical microscopy and super-resolution techniques.

Read More

[IMG: JPK NanoRacer]
[H4] NanoRacer High-Speed AFM
True high-speed imaging at 50 frames and 5000 lines per second and exceptional usability for following molecular dynamics in real-time.

Mehr

[IMG: Dimension FastScan Bio]
[H4] Dimension FastScan Bio
FastScan speeds and superior AFM ease of use for life science

Mehr

[H3] BioAFMs for Cells and Tissues

[H4] NEW CellWizard Stage
Automated, AI-guided, multi-chamber in vitro imaging and nanomechanical analysis.

READ MORE

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[IMG: CellHesion 300 - automated, quantitative single-cell and tissue mechanics]
[H4] CellHesion 300
Automated platform for measuring cell-cell, cell-tissue, and cell-substrate interactions with single-molecule sensitivity.

Mehr

[H3] BioAFMs for Single-Molecule Applications

[IMG: ForceRobot® 400 Automated Force Spectroscope]
[H4] ForceRobot 400
For single molecule applications. Automated force spectroscope with optional temperature control and fluidics system.

Mehr

[H3] Related Technologies

[IMG: Optical Tweezers]
[H4] Optical Tweezers
Force-sensing optical tweezers and optical trapping

Mehr

Accessories and System Options
[H2] Customize Your BioAFM System

[H3]
Accessories to extend your BioAFM capabilities
Bruker offers an extensive range of BioAFM system add-ons, accessories, and modes to deliver maximum experimental and sample control, superior versatility, and enhanced useability. These options extend the range of applications and experiments supported by Bruker BioAFMs far beyond what is possible with any other BioAFM system on the market today.
Available options include optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions, and more.

Browse our online accessories database or download our accessories handbook to learn more.

[IMG: Options and add-ons for Bruker]
[H4] BioAFM Accessories Handbook [PDF]
Learn more about options and add-ons for Bruker BioAFMs, including 100+ accessories and 30 modes.

Download Now

[H2] Explore Our Virtual Showroom

BioAFM FAQs
[H2] Frequently Asked Questions

BioAFM is an increasingly important tool in biological and biomedical studies due to its very high resolution and the possibility of conducting experiments with live cells under physiologically relevant and ambient conditions, even in liquid. BioAFM also provides nanometer-resolution surface mapping for many mechanical and electrical properties, such as elasticity, stiffness, conductivity and surface potential. Bruker BioAFM technology is enabling life science researchers to investigate how these properties impact on cellular functions, including communication, signaling, cell division and differentiation, and even tumor metastasis and infection.

What is a BioAFM?

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter. BioAFM instruments can measure soft, fragile, and challenging samples — including single molecules, nucleic acids, bacteria, living cells, and tissues — under near-physiological conditions without damaging their structures. These instruments are uniquely capable of performing non-invasive measurements on biological samples and label-free measurements in liquid.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

How are BioAFMs specifically adapted for biological studies?

[H4] How are BioAFMs specifically adapted for biological studies?
BioAFMs have specialized sample and cantilever holders, stages, and measurement modes specifically designed for life science samples and experiments. Bruker BioAFM instruments also can be uniquely configured with a large selection of optional accessories and advanced modes, extending sample compatibility and measurement capabilities to include even the most challenging soft matter and biological samples.
Unique features and capabilities of Bruker BioAFM instruments include:
High-speed AFM with up to 50 frames per second enables the visualization of molecular dynamics.Precise force control prevents damage of delicate biological samples.Fully integrated correlated analysis with a wide range of advanced optical techniques.Data analysis methods for soft samples (e.g., contact point imaging).Large z range (>100µm) for measuring challenging biological samples with steep, highly corrugated surfaces, and cell adhesion experiments.Advanced automation features (e.g., detector alignment, creation of experiment workflows, and adjustment of scanning parameters) enable self-regulating measurement routines that maximize throughput and improve accuracy.Optical Tiling software feature for navigating around large samples.Cantilever holders that can be cleaned and autoclaved, providing direct access to biohazards in biosafety laboratories and allowing sample preparation, loading, experiments, and disposal to be performed in the BSL facility.Accessories for investigating a diverse range of biological and soft matter samples on substrates of various sizes, shapes, and materials (e.g., Petri dishes, coverslips, biochips, etc.) and in different environmental conditions (e.g., atmosphere, temperature, gas, pH-value, etc.).Automated, large area, multiparametric characterization of densely packed cell layers and highly corrugated tissue samples without microtome cutting.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

Browse All BioAFM Accessories

What are the advantages of using atomic force microscopy for biological research?

[H4] What are the advantages of using a BioAFM?
Atomic force microscopy provides 3D images of surface features and topography. BioAFMs extend and enhance the technique, optimizing it to address the unique needs and challenges of biological research and providing distinct advantages over other methods. Key advantages include:
High spatiotemporal resolution: Investigate morphology/surface structure at sub-molecular resolution and interactions in the piconewton range.AFM in liquids and environmental control: Study samples in liquid and under near-physiological conditions — ideal for the investigation of living cells in medium — capabilities that are unique to atomic force microscopy.Label-free and non-invasive investigation of living cells and delicate biological samples.Easy combination with other advanced optical microscopy techniques for correlated measurements and complementary datasets that provide a more comprehensive insight into biological processes.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

What are the advantages of automated BioAFM measurements in biological experiments?

[H4] What are the advantages of automated BioAFM measurements?
Bruker BioAFMs feature several specialized software functions and capabilities designed to support high-performance AFM-based investigation of biological samples and soft matter. Automated instrument procedures (e.g., alignment, operation, calibration), measurement routines, and data analysis capabilities make it easier to set up and run experiments while also improving the accuracy and reproducibility of results.
Advanced automation features include:
ExperimentPlanner: Predefinition of settings and parameters allows complex experiments to run automatically.ExperimentControl: Remote monitoring of long-term lab experiments via a browser on any device. Together with ExperimentPlanner, these features open a host of new possibilities for long-term, self-regulating experiment series that reflect “real-life” conditions. This alleviates long and repetitive experiments and gives researchers the freedom to oversee and control their experiments remotely.SmartMapping: The novel SmartMapping feature allows the flexible selection of multiple, user-defined 2D force maps. Numerous regions of interest (ROI) can be selected in advance and examined automatically, enabling the systematic study of large sample areas. Automated large-area, multi-region imaging (by combining DirectTiling, DirectOverlay 2, and MultiScan software features) extends the optical viewing field. Perfect optical integration enables direct correlation of AFM and optical data.
These features extend and enhance the capabilities of Bruker BioAFM systems in order to:
Increase throughput and productivity.Produce statistically relevant datasets.Reveal parameter correlation and cross-correlation via automated cycling through of parameters.Allow unattended, long term, self-regulating experiment series.Enable remote monitoring of long-term lab experiments.
The improved throughput, standardized batch analysis routines, and statistically relevant datasets generated by these types of automated features are essential in biological research, particularly in nanomedical and clinical fields.

RELATED LINKS:

[WATCH] Automated AFM and Applications in Mechanobiology and Life Science Research

Browse our Automated BioAFM Accessories

How and why do you integrate an AFM with an optical/fluorescence microscope?

[H4] How and why do you integrate a BioAFM with an optical/fluorescence microscope?
A major advantage of Bruker’s BioAFM instrumentation is that they can be easily combined with advanced optical microscopy techniques, e.g., fluorescence or STED microscopy, for correlated measurements and complementary datasets.
Our BioAFMs can be easily combined with advanced optical imaging techniques by using a specific AFM stage that is compatible with most commercially available inverse and confocal optical microscopes. The AFM head is placed on the stage and software, such as Bruker’s DirectOverlay feature, enables colocalization of the optical and AFM images and direct correlation of the AFM and optical data. Transferring the sample between setups is not necessary and a wide range of camera and detector types are supported. Easy optical image import, advanced calibration algorithms, and visualization routines facilitate accurate navigation on and around the sample, providing multidimensional sample characterization in a single experiment. Compatible techniques include epifluorescence, confocal, phase contrast, and super-resolution microscopy techniques (STED, TIRF, STORM).
The ability to obtain real-time, correlative data sets is particularly relevant in life science research because it:
Allows researchers to study the topography of biological samples using AFM while simultaneously observing fluorescently labeled cellular components; andEnables the multiparametric observation of in-situ dynamics, such as single-molecule protein dynamics, protein folding, receptor-ligand interactions, and mechanosensitive signaling pathways.

RELATED LINKS:

[WATC

Microscopes
[H1] Life Sciences Research AFMs

Providing unique insights into life sciences research with previously inaccessible data, including physical structure, biochemical interactions, and mechanical properties

[H2] Advanced Imaging

Bruker has been helping biologists discover, understand, publish, and advance their knowledge of biological systems ever since our introduction of TappingMode™ expanded AFM use into life sciences research. In addition to general-purpose BioAFM systems specifically designed for life sciences research and suitable for a broad range of applications, Bruker offers a variety of specialized systems utilizing proprietary AFM features. These instruments include BioAFMs for high-speed imaging to support the investigation of dynamic processes; BioAFMs optimized for the investigation of cells and tissues, even uneven or highly corrugated samples; and BioAFMs for single-molecule applications ranging from single-molecule property characterization to the investigation of conformational changes at the single-molecule level, intra-molecular dynamics, and single-molecule spectroscopy (SMFS).
These instruments deliver the highest quality results for quantitative live-cell mechanical property mapping, highest resolution molecular and cellular imaging, and fast scanning of dynamic biological processes, as well as seamless integration of AFM with advanced optical microscopy techniques.

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter.

Learn More About BioAFM Technology and Capabilities

[H4] AFM for Life Science Research E-Book
Learn more about BioAFM technology and get practical tips and other information valuable to both AFM users and those not familiar with the technique.

Download the E-Book

[H4] Browse All BioAFM Resources
Explore our extensive offering of resources, including a brand new poster featuring the NanoWizard AFM platform, application and technical notes, research highlights, and more.

Learn More

Products
[H2] Find the Best BioAFM for Your Application

[H3] General-Purpose BioAFMs

[H4] NEW NanoWizard PURE
Streamlined design with best-in-class performance and flexibility.

Read More

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[H3] BioAFMs for High-Speed Imaging

[H4] NEW NanoWizard ULTRA Speed 3
Fast automated imaging at 1,400 lines per second and seamless integration into advanced optical microscopy and super-resolution techniques.

Read More

[IMG: JPK NanoRacer]
[H4] NanoRacer High-Speed AFM
True high-speed imaging at 50 frames and 5000 lines per second and exceptional usability for following molecular dynamics in real-time.

Mehr

[IMG: Dimension FastScan Bio]
[H4] Dimension FastScan Bio
FastScan speeds and superior AFM ease of use for life science

Mehr

[H3] BioAFMs for Cells and Tissues

[H4] NEW CellWizard Stage
Automated, AI-guided, multi-chamber in vitro imaging and nanomechanical analysis.

READ MORE

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[IMG: CellHesion 300 - automated, quantitative single-cell and tissue mechanics]
[H4] CellHesion 300
Automated platform for measuring cell-cell, cell-tissue, and cell-substrate interactions with single-molecule sensitivity.

Mehr

[H3] BioAFMs for Single-Molecule Applications

[IMG: ForceRobot® 400 Automated Force Spectroscope]
[H4] ForceRobot 400
For single molecule applications. Automated force spectroscope with optional temperature control and fluidics system.

Mehr

[H3] Related Technologies

[IMG: Optical Tweezers]
[H4] Optical Tweezers
Force-sensing optical tweezers and optical trapping

Mehr

Accessories and System Options
[H2] Customize Your BioAFM System

[H3]
Accessories to extend your BioAFM capabilities
Bruker offers an extensive range of BioAFM system add-ons, accessories, and modes to deliver maximum experimental and sample control, superior versatility, and enhanced useability. These options extend the range of applications and experiments supported by Bruker BioAFMs far beyond what is possible with any other BioAFM system on the market today.
Available options include optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions, and more.

Browse our online accessories database or download our accessories handbook to learn more.

[IMG: Options and add-ons for Bruker]
[H4] BioAFM Accessories Handbook [PDF]
Learn more about options and add-ons for Bruker BioAFMs, including 100+ accessories and 30 modes.

Download Now

[H2] Explore Our Virtual Showroom

BioAFM FAQs
[H2] Frequently Asked Questions

BioAFM is an increasingly important tool in biological and biomedical studies due to its very high resolution and the possibility of conducting experiments with live cells under physiologically relevant and ambient conditions, even in liquid. BioAFM also provides nanometer-resolution surface mapping for many mechanical and electrical properties, such as elasticity, stiffness, conductivity and surface potential. Bruker BioAFM technology is enabling life science researchers to investigate how these properties impact on cellular functions, including communication, signaling, cell division and differentiation, and even tumor metastasis and infection.

What is a BioAFM?

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter. BioAFM instruments can measure soft, fragile, and challenging samples — including single molecules, nucleic acids, bacteria, living cells, and tissues — under near-physiological conditions without damaging their structures. These instruments are uniquely capable of performing non-invasive measurements on biological samples and label-free measurements in liquid.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

How are BioAFMs specifically adapted for biological studies?

[H4] How are BioAFMs specifically adapted for biological studies?
BioAFMs have specialized sample and cantilever holders, stages, and measurement modes specifically designed for life science samples and experiments. Bruker BioAFM instruments also can be uniquely configured with a large selection of optional accessories and advanced modes, extending sample compatibility and measurement capabilities to include even the most challenging soft matter and biological samples.
Unique features and capabilities of Bruker BioAFM instruments include:
High-speed AFM with up to 50 frames per second enables the visualization of molecular dynamics.Precise force control prevents damage of delicate biological samples.Fully integrated correlated analysis with a wide range of advanced optical techniques.Data analysis methods for soft samples (e.g., contact point imaging).Large z range (>100µm) for measuring challenging biological samples with steep, highly corrugated surfaces, and cell adhesion experiments.Advanced automation features (e.g., detector alignment, creation of experiment workflows, and adjustment of scanning parameters) enable self-regulating measurement routines that maximize throughput and improve accuracy.Optical Tiling software feature for navigating around large samples.Cantilever holders that can be cleaned and autoclaved, providing direct access to biohazards in biosafety laboratories and allowing sample preparation, loading, experiments, and disposal to be performed in the BSL facility.Accessories for investigating a diverse range of biological and soft matter samples on substrates of various sizes, shapes, and materials (e.g., Petri dishes, coverslips, biochips, etc.) and in different environmental conditions (e.g., atmosphere, temperature, gas, pH-value, etc.).Automated, large area, multiparametric characterization of densely packed cell layers and highly corrugated tissue samples without microtome cutting.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

Browse All BioAFM Accessories

What are the advantages of using atomic force microscopy for biological research?

[H4] What are the advantages of using a BioAFM?
Atomic force microscopy provides 3D images of surface features and topography. BioAFMs extend and enhance the technique, optimizing it to address the unique needs and challenges of biological research and providing distinct advantages over other methods. Key advantages include:
High spatiotemporal resolution: Investigate morphology/surface structure at sub-molecular resolution and interactions in the piconewton range.AFM in liquids and environmental control: Study samples in liquid and under near-physiological conditions — ideal for the investigation of living cells in medium — capabilities that are unique to atomic force microscopy.Label-free and non-invasive investigation of living cells and delicate biological samples.Easy combination with other advanced optical microscopy techniques for correlated measurements and complementary datasets that provide a more comprehensive insight into biological processes.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

What are the advantages of automated BioAFM measurements in biological experiments?

[H4] What are the advantages of automated BioAFM measurements?
Bruker BioAFMs feature several specialized software functions and capabilities designed to support high-performance AFM-based investigation of biological samples and soft matter. Automated instrument procedures (e.g., alignment, operation, calibration), measurement routines, and data analysis capabilities make it easier to set up and run experiments while also improving the accuracy and reproducibility of results.
Advanced automation features include:
ExperimentPlanner: Predefinition of settings and parameters allows complex experiments to run automatically.ExperimentControl: Remote monitoring of long-term lab experiments via a browser on any device. Together with ExperimentPlanner, these features open a host of new possibilities for long-term, self-regulating experiment series that reflect “real-life” conditions. This alleviates long and repetitive experiments and gives researchers the freedom to oversee and control their experiments remotely.SmartMapping: The novel SmartMapping feature allows the flexible selection of multiple, user-defined 2D force maps. Numerous regions of interest (ROI) can be selected in advance and examined automatically, enabling the systematic study of large sample areas. Automated large-area, multi-region imaging (by combining DirectTiling, DirectOverlay 2, and MultiScan software features) extends the optical viewing field. Perfect optical integration enables direct correlation of AFM and optical data.
These features extend and enhance the capabilities of Bruker BioAFM systems in order to:
Increase throughput and productivity.Produce statistically relevant datasets.Reveal parameter correlation and cross-correlation via automated cycling through of parameters.Allow unattended, long term, self-regulating experiment series.Enable remote monitoring of long-term lab experiments.
The improved throughput, standardized batch analysis routines, and statistically relevant datasets generated by these types of automated features are essential in biological research, particularly in nanomedical and clinical fields.

RELATED LINKS:

[WATCH] Automated AFM and Applications in Mechanobiology and Life Science Research

Browse our Automated BioAFM Accessories

How and why do you integrate an AFM with an optical/fluorescence microscope?

[H4] How and why do you integrate a BioAFM with an optical/fluorescence microscope?
A major advantage of Bruker’s BioAFM instrumentation is that they can be easily combined with advanced optical microscopy techniques, e.g., fluorescence or STED microscopy, for correlated measurements and complementary datasets.
Our BioAFMs can be easily combined with advanced optical imaging techniques by using a specific AFM stage that is compatible with most commercially available inverse and confocal optical microscopes. The AFM head is placed on the stage and software, such as Bruker’s DirectOverlay feature, enables colocalization of the optical and AFM images and direct correlation of the AFM and optical data. Transferring the sample between setups is not necessary and a wide range of camera and detector types are supported. Easy optical image import, advanced calibration algorithms, and visualization routines facilitate accurate navigation on and around the sample, providing multidimensional sample characterization in a single experiment. Compatible techniques include epifluorescence, confocal, phase contrast, and super-resolution microscopy techniques (STED, TIRF, STORM).
The ability to obtain real-time, correlative data sets is particularly relevant in life science research because it:
Allows researchers to study the topography of biological samples using AFM while simultaneously observing fluorescently labeled cellular components; andEnables the multiparametric observation of in-situ dynamics, such as single-molecule protein dynamics, protein folding, receptor-ligand interactions, and mechanosensitive signaling pathways.

RELATED LINKS:

[WATC

Microscopes
[H1] Life Sciences Research AFMs

Providing unique insights into life sciences research with previously inaccessible data, including physical structure, biochemical interactions, and mechanical properties

[H2] Advanced Imaging

Bruker has been helping biologists discover, understand, publish, and advance their knowledge of biological systems ever since our introduction of TappingMode™ expanded AFM use into life sciences research. In addition to general-purpose BioAFM systems specifically designed for life sciences research and suitable for a broad range of applications, Bruker offers a variety of specialized systems utilizing proprietary AFM features. These instruments include BioAFMs for high-speed imaging to support the investigation of dynamic processes; BioAFMs optimized for the investigation of cells and tissues, even uneven or highly corrugated samples; and BioAFMs for single-molecule applications ranging from single-molecule property characterization to the investigation of conformational changes at the single-molecule level, intra-molecular dynamics, and single-molecule spectroscopy (SMFS).
These instruments deliver the highest quality results for quantitative live-cell mechanical property mapping, highest resolution molecular and cellular imaging, and fast scanning of dynamic biological processes, as well as seamless integration of AFM with advanced optical microscopy techniques.

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter.

Learn More About BioAFM Technology and Capabilities

[H4] AFM for Life Science Research E-Book
Learn more about BioAFM technology and get practical tips and other information valuable to both AFM users and those not familiar with the technique.

Download the E-Book

[H4] Browse All BioAFM Resources
Explore our extensive offering of resources, including a brand new poster featuring the NanoWizard AFM platform, application and technical notes, research highlights, and more.

Learn More

Products
[H2] Find the Best BioAFM for Your Application

[H3] General-Purpose BioAFMs

[H4] NEW NanoWizard PURE
Streamlined design with best-in-class performance and flexibility.

Read More

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[H3] BioAFMs for High-Speed Imaging

[H4] NEW NanoWizard ULTRA Speed 3
Fast automated imaging at 1,400 lines per second and seamless integration into advanced optical microscopy and super-resolution techniques.

Read More

[IMG: JPK NanoRacer]
[H4] NanoRacer High-Speed AFM
True high-speed imaging at 50 frames and 5000 lines per second and exceptional usability for following molecular dynamics in real-time.

Mehr

[IMG: Dimension FastScan Bio]
[H4] Dimension FastScan Bio
FastScan speeds and superior AFM ease of use for life science

Mehr

[H3] BioAFMs for Cells and Tissues

[H4] NEW CellWizard Stage
Automated, AI-guided, multi-chamber in vitro imaging and nanomechanical analysis.

READ MORE

[IMG: NanoWizard 5 AFM]
[H4] NanoWizard V BioScience
Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

Mehr

[IMG: NanoWizard 4 XP BioScience AFM]
[H4] NanoWizard 4 XP BioScience
Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

Mehr

[IMG: CellHesion 300 - automated, quantitative single-cell and tissue mechanics]
[H4] CellHesion 300
Automated platform for measuring cell-cell, cell-tissue, and cell-substrate interactions with single-molecule sensitivity.

Mehr

[H3] BioAFMs for Single-Molecule Applications

[IMG: ForceRobot® 400 Automated Force Spectroscope]
[H4] ForceRobot 400
For single molecule applications. Automated force spectroscope with optional temperature control and fluidics system.

Mehr

[H3] Related Technologies

[IMG: Optical Tweezers]
[H4] Optical Tweezers
Force-sensing optical tweezers and optical trapping

Mehr

Accessories and System Options
[H2] Customize Your BioAFM System

[H3]
Accessories to extend your BioAFM capabilities
Bruker offers an extensive range of BioAFM system add-ons, accessories, and modes to deliver maximum experimental and sample control, superior versatility, and enhanced useability. These options extend the range of applications and experiments supported by Bruker BioAFMs far beyond what is possible with any other BioAFM system on the market today.
Available options include optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions, and more.

Browse our online accessories database or download our accessories handbook to learn more.

[IMG: Options and add-ons for Bruker]
[H4] BioAFM Accessories Handbook [PDF]
Learn more about options and add-ons for Bruker BioAFMs, including 100+ accessories and 30 modes.

Download Now

[H2] Explore Our Virtual Showroom

BioAFM FAQs
[H2] Frequently Asked Questions

BioAFM is an increasingly important tool in biological and biomedical studies due to its very high resolution and the possibility of conducting experiments with live cells under physiologically relevant and ambient conditions, even in liquid. BioAFM also provides nanometer-resolution surface mapping for many mechanical and electrical properties, such as elasticity, stiffness, conductivity and surface potential. Bruker BioAFM technology is enabling life science researchers to investigate how these properties impact on cellular functions, including communication, signaling, cell division and differentiation, and even tumor metastasis and infection.

What is a BioAFM?

[H4] What is a BioAFM?
A BioAFM is an atomic force microscope specifically adapted for studying biological samples and soft matter. BioAFM instruments can measure soft, fragile, and challenging samples — including single molecules, nucleic acids, bacteria, living cells, and tissues — under near-physiological conditions without damaging their structures. These instruments are uniquely capable of performing non-invasive measurements on biological samples and label-free measurements in liquid.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

How are BioAFMs specifically adapted for biological studies?

[H4] How are BioAFMs specifically adapted for biological studies?
BioAFMs have specialized sample and cantilever holders, stages, and measurement modes specifically designed for life science samples and experiments. Bruker BioAFM instruments also can be uniquely configured with a large selection of optional accessories and advanced modes, extending sample compatibility and measurement capabilities to include even the most challenging soft matter and biological samples.
Unique features and capabilities of Bruker BioAFM instruments include:
High-speed AFM with up to 50 frames per second enables the visualization of molecular dynamics.Precise force control prevents damage of delicate biological samples.Fully integrated correlated analysis with a wide range of advanced optical techniques.Data analysis methods for soft samples (e.g., contact point imaging).Large z range (>100µm) for measuring challenging biological samples with steep, highly corrugated surfaces, and cell adhesion experiments.Advanced automation features (e.g., detector alignment, creation of experiment workflows, and adjustment of scanning parameters) enable self-regulating measurement routines that maximize throughput and improve accuracy.Optical Tiling software feature for navigating around large samples.Cantilever holders that can be cleaned and autoclaved, providing direct access to biohazards in biosafety laboratories and allowing sample preparation, loading, experiments, and disposal to be performed in the BSL facility.Accessories for investigating a diverse range of biological and soft matter samples on substrates of various sizes, shapes, and materials (e.g., Petri dishes, coverslips, biochips, etc.) and in different environmental conditions (e.g., atmosphere, temperature, gas, pH-value, etc.).Automated, large area, multiparametric characterization of densely packed cell layers and highly corrugated tissue samples without microtome cutting.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

Browse All BioAFM Accessories

What are the advantages of using atomic force microscopy for biological research?

[H4] What are the advantages of using a BioAFM?
Atomic force microscopy provides 3D images of surface features and topography. BioAFMs extend and enhance the technique, optimizing it to address the unique needs and challenges of biological research and providing distinct advantages over other methods. Key advantages include:
High spatiotemporal resolution: Investigate morphology/surface structure at sub-molecular resolution and interactions in the piconewton range.AFM in liquids and environmental control: Study samples in liquid and under near-physiological conditions — ideal for the investigation of living cells in medium — capabilities that are unique to atomic force microscopy.Label-free and non-invasive investigation of living cells and delicate biological samples.Easy combination with other advanced optical microscopy techniques for correlated measurements and complementary datasets that provide a more comprehensive insight into biological processes.

RELATED LINKS:

E-Book: Atomic Force Microscopy for Life Sciences

[WATCH] BioAFM Office Hours: Explore the World of BioAFM with Bruker's Experts

What are the advantages of automated BioAFM measurements in biological experiments?

[H4] What are the advantages of automated BioAFM measurements?
Bruker BioAFMs feature several specialized software functions and capabilities designed to support high-performance AFM-based investigation of biological samples and soft matter. Automated instrument procedures (e.g., alignment, operation, calibration), measurement routines, and data analysis capabilities make it easier to set up and run experiments while also improving the accuracy and reproducibility of results.
Advanced automation features include:
ExperimentPlanner: Predefinition of settings and parameters allows complex experiments to run automatically.ExperimentControl: Remote monitoring of long-term lab experiments via a browser on any device. Together with ExperimentPlanner, these features open a host of new possibilities for long-term, self-regulating experiment series that reflect “real-life” conditions. This alleviates long and repetitive experiments and gives researchers the freedom to oversee and control their experiments remotely.SmartMapping: The novel SmartMapping feature allows the flexible selection of multiple, user-defined 2D force maps. Numerous regions of interest (ROI) can be selected in advance and examined automatically, enabling the systematic study of large sample areas. Automated large-area, multi-region imaging (by combining DirectTiling, DirectOverlay 2, and MultiScan software features) extends the optical viewing field. Perfect optical integration enables direct correlation of AFM and optical data.
These features extend and enhance the capabilities of Bruker BioAFM systems in order to:
Increase throughput and productivity.Produce statistically relevant datasets.Reveal parameter correlation and cross-correlation via automated cycling through of parameters.Allow unattended, long term, self-regulating experiment series.Enable remote monitoring of long-term lab experiments.
The improved throughput, standardized batch analysis routines, and statistically relevant datasets generated by these types of automated features are essential in biological research, particularly in nanomedical and clinical fields.

RELATED LINKS:

[WATCH] Automated AFM and Applications in Mechanobiology and Life Science Research

Browse our Automated BioAFM Accessories

How and why do you integrate an AFM with an optical/fluorescence microscope?

[H4] How and why do you integrate a BioAFM with an optical/fluorescence microscope?
A major advantage of Bruker’s BioAFM instrumentation is that they can be easily combined with advanced optical microscopy techniques, e.g., fluorescence or STED microscopy, for correlated measurements and complementary datasets.
Our BioAFMs can be easily combined with advanced optical imaging techniques by using a specific AFM stage that is compatible with most commercially available inverse and confocal optical microscopes. The AFM head is placed on the stage and software, such as Bruker’s DirectOverlay feature, enables colocalization of the optical and AFM images and direct correlation of the AFM and optical data. Transferring the sample between setups is not necessary and a wide range of camera and detector types are supported. Easy optical image import, advanced calibration algorithms, and visualization routines facilitate accurate navigation on and around the sample, providing multidimensional sample characterization in a single experiment. Compatible techniques include epifluorescence, confocal, phase contrast, and super-resolution microscopy techniques (STED, TIRF, STORM).
The ability to obtain real-time, correlative data sets is particularly relevant in life science research because it:
Allows researchers to study the topography of biological samples using AFM while simultaneously observing fluorescently labeled cellular components; andEnables the multiparametric observation of in-situ dynamics, such as single-molecule protein dynamics, protein folding, receptor-ligand interactions, and mechanosensitive signaling pathways.

RELATED LINKS:

[WATC